Sequencing sample homology detection method and system based on small sample SNPs linear fitting

The invention discloses a sequencing sample homology detection method and system based on small sample SNPs linear fitting, and belongs to the technical field of sequencing sample detection. The method comprises the steps of obtaining a plurality of gene files; respectively filtering and screening the SNPs in the plurality of gene files according to a predetermined filtering and screening condition to obtain the filtered and screened SNPs; calculating the mutation allele frequency of each SNPs after filtering and screening; performing linear fitting based on the mutation allele frequencies of any two samples, and calculating first, second and third linear fitting parameters; and determining whether the sequencing sample is homologous based on the first, second and third linear fitting parameters. The invention further discloses a corresponding system, only two samples need to use the same sequencing method or a large number of overlapped SNPs exist between the two samples, the VCF files generated in the NGS s