Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine

Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine

The invention discloses a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and a kit for detecting 11-dehydrothromboxane B2 (11-dehydrothromboxane B2, 11dhTxB2) in urine, and the liquid chromatography-tandem mass spectrometry method for detecting 11dhTxB2 in urine comprises the follo...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: ZHANG YALIAN, XU JING, CUI YING, ZHAO FURONG, SUN XIAOYU, CAO YUNFENG, WANG SHUANG
Format: Patent
Sprache:chi ; eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume
creator ZHANG YALIAN
XU JING
CUI YING
ZHAO FURONG
SUN XIAOYU
CAO YUNFENG
WANG SHUANG
description The invention discloses a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and a kit for detecting 11-dehydrothromboxane B2 (11-dehydrothromboxane B2, 11dhTxB2) in urine, and the liquid chromatography-tandem mass spectrometry method for detecting 11dhTxB2 in urine comprises the following steps: (1) adding a stabilizing agent into a urine sample, adjusting the pH value with an acidifying agent, incubating for a period of time, and detecting the content of 11dhTxB2 in the urine sample; the method comprises the following steps: extracting 11dhTxB2, adding an isotope internal standard, performing impurity removal, purification and enrichment on 11dhTxB2 through a specific solid-phase extraction column, and analyzing a sample extracting solution by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). And (2) a kit for detecting 11dhTxB2 in urine, wherein the kit comprises a specific solid phase extraction column, an internal standard solution, a calibration solution, a diluent, a stabil
format Patent
fullrecord <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN115656400A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN115656400A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN115656400A3</originalsourceid><addsrcrecordid>eNqNjDsOwjAQBdNQIOAOywEsxUDSQwSiQFT00RJvYgv8wd5I5PYYiQPQvClm9OaFvZjXaBR0OnqL7IeIQU-C0SmyYDElSIE6zpY4TpBXewVZw8Mw9D6CIs6BcQNIKRTpSUXP37u7f6MjOGzAOBijcbQsZj0-E61-XBTr0_HWnAUF31IK2JEjbpurlFVd1buy3G__aT6ixUI0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine</title><source>esp@cenet</source><creator>ZHANG YALIAN ; XU JING ; CUI YING ; ZHAO FURONG ; SUN XIAOYU ; CAO YUNFENG ; WANG SHUANG</creator><creatorcontrib>ZHANG YALIAN ; XU JING ; CUI YING ; ZHAO FURONG ; SUN XIAOYU ; CAO YUNFENG ; WANG SHUANG</creatorcontrib><description>The invention discloses a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and a kit for detecting 11-dehydrothromboxane B2 (11-dehydrothromboxane B2, 11dhTxB2) in urine, and the liquid chromatography-tandem mass spectrometry method for detecting 11dhTxB2 in urine comprises the following steps: (1) adding a stabilizing agent into a urine sample, adjusting the pH value with an acidifying agent, incubating for a period of time, and detecting the content of 11dhTxB2 in the urine sample; the method comprises the following steps: extracting 11dhTxB2, adding an isotope internal standard, performing impurity removal, purification and enrichment on 11dhTxB2 through a specific solid-phase extraction column, and analyzing a sample extracting solution by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). And (2) a kit for detecting 11dhTxB2 in urine, wherein the kit comprises a specific solid phase extraction column, an internal standard solution, a calibration solution, a diluent, a stabil</description><language>chi ; eng</language><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; PHYSICS ; TESTING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20230131&amp;DB=EPODOC&amp;CC=CN&amp;NR=115656400A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25562,76317</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&amp;date=20230131&amp;DB=EPODOC&amp;CC=CN&amp;NR=115656400A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHANG YALIAN</creatorcontrib><creatorcontrib>XU JING</creatorcontrib><creatorcontrib>CUI YING</creatorcontrib><creatorcontrib>ZHAO FURONG</creatorcontrib><creatorcontrib>SUN XIAOYU</creatorcontrib><creatorcontrib>CAO YUNFENG</creatorcontrib><creatorcontrib>WANG SHUANG</creatorcontrib><title>Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine</title><description>The invention discloses a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and a kit for detecting 11-dehydrothromboxane B2 (11-dehydrothromboxane B2, 11dhTxB2) in urine, and the liquid chromatography-tandem mass spectrometry method for detecting 11dhTxB2 in urine comprises the following steps: (1) adding a stabilizing agent into a urine sample, adjusting the pH value with an acidifying agent, incubating for a period of time, and detecting the content of 11dhTxB2 in the urine sample; the method comprises the following steps: extracting 11dhTxB2, adding an isotope internal standard, performing impurity removal, purification and enrichment on 11dhTxB2 through a specific solid-phase extraction column, and analyzing a sample extracting solution by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). And (2) a kit for detecting 11dhTxB2 in urine, wherein the kit comprises a specific solid phase extraction column, an internal standard solution, a calibration solution, a diluent, a stabil</description><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>PHYSICS</subject><subject>TESTING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjDsOwjAQBdNQIOAOywEsxUDSQwSiQFT00RJvYgv8wd5I5PYYiQPQvClm9OaFvZjXaBR0OnqL7IeIQU-C0SmyYDElSIE6zpY4TpBXewVZw8Mw9D6CIs6BcQNIKRTpSUXP37u7f6MjOGzAOBijcbQsZj0-E61-XBTr0_HWnAUF31IK2JEjbpurlFVd1buy3G__aT6ixUI0</recordid><startdate>20230131</startdate><enddate>20230131</enddate><creator>ZHANG YALIAN</creator><creator>XU JING</creator><creator>CUI YING</creator><creator>ZHAO FURONG</creator><creator>SUN XIAOYU</creator><creator>CAO YUNFENG</creator><creator>WANG SHUANG</creator><scope>EVB</scope></search><sort><creationdate>20230131</creationdate><title>Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine</title><author>ZHANG YALIAN ; XU JING ; CUI YING ; ZHAO FURONG ; SUN XIAOYU ; CAO YUNFENG ; WANG SHUANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115656400A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>PHYSICS</topic><topic>TESTING</topic><toplevel>online_resources</toplevel><creatorcontrib>ZHANG YALIAN</creatorcontrib><creatorcontrib>XU JING</creatorcontrib><creatorcontrib>CUI YING</creatorcontrib><creatorcontrib>ZHAO FURONG</creatorcontrib><creatorcontrib>SUN XIAOYU</creatorcontrib><creatorcontrib>CAO YUNFENG</creatorcontrib><creatorcontrib>WANG SHUANG</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ZHANG YALIAN</au><au>XU JING</au><au>CUI YING</au><au>ZHAO FURONG</au><au>SUN XIAOYU</au><au>CAO YUNFENG</au><au>WANG SHUANG</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine</title><date>2023-01-31</date><risdate>2023</risdate><abstract>The invention discloses a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and a kit for detecting 11-dehydrothromboxane B2 (11-dehydrothromboxane B2, 11dhTxB2) in urine, and the liquid chromatography-tandem mass spectrometry method for detecting 11dhTxB2 in urine comprises the following steps: (1) adding a stabilizing agent into a urine sample, adjusting the pH value with an acidifying agent, incubating for a period of time, and detecting the content of 11dhTxB2 in the urine sample; the method comprises the following steps: extracting 11dhTxB2, adding an isotope internal standard, performing impurity removal, purification and enrichment on 11dhTxB2 through a specific solid-phase extraction column, and analyzing a sample extracting solution by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). And (2) a kit for detecting 11dhTxB2 in urine, wherein the kit comprises a specific solid phase extraction column, an internal standard solution, a calibration solution, a diluent, a stabil</abstract><oa>free_for_read</oa></addata></record>
fulltext fulltext_linktorsrc
identifier
ispartof
issn
language chi ; eng
recordid cdi_epo_espacenet_CN115656400A
source esp@cenet
subjects INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
title Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T06%3A24%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=ZHANG%20YALIAN&rft.date=2023-01-31&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN115656400A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true