Liquid chromatography-tandem mass spectrometry method and kit for detecting 11-dehydrothromboxane B2 in urine

The invention discloses a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and a kit for detecting 11-dehydrothromboxane B2 (11-dehydrothromboxane B2, 11dhTxB2) in urine, and the liquid chromatography-tandem mass spectrometry method for detecting 11dhTxB2 in urine comprises the following steps: (1) adding a stabilizing agent into a urine sample, adjusting the pH value with an acidifying agent, incubating for a period of time, and detecting the content of 11dhTxB2 in the urine sample; the method comprises the following steps: extracting 11dhTxB2, adding an isotope internal standard, performing impurity removal, purification and enrichment on 11dhTxB2 through a specific solid-phase extraction column, and analyzing a sample extracting solution by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). And (2) a kit for detecting 11dhTxB2 in urine, wherein the kit comprises a specific solid phase extraction column, an internal standard solution, a calibration solution, a diluent, a stabil