Preparation method of DC-CIK cell treatment composition

The invention discloses a preparation method of a DC-CIK cell treatment composition, which comprises the following steps: firstly obtaining cells, obtaining DC and CIK cells through umbilical cord blood mononuclear cells, respectively centrifuging the obtained DC and CIK cells, respectively cleaning the DC and CIK cells by using normal saline after centrifugation, and then carrying out DC cell culture; a basic nutrient solution, CM-SCF, insulin, IL-6, IL-10 and IL-15 are added, and the DC cells are cultured for 3 days; the PAD14 protein is adopted as a catalyst to catalyze the DC-CIK cells, the maturation speed of the cells can be increased, the DC-CIK cells are subjected to combined culture through a stimulating factor auxiliary catalyst, the cells can be effectively stimulated, the cell activity is enhanced, the proliferation and development speed of the cells is increased through the activity of high-strength active cells, and the cell proliferation and development speed is increased. And the purpose of sh