Preparation method of cell preserving fluid and prepared cell preserving fluid

The invention relates to the technical field of biology, in particular to a preparation method of a cell preserving fluid and the prepared cell preserving fluid. The invention aims to provide a method for preparing a cell preservation solution, and the cell preservation solution which can maintain cell activity during long-term transportation and can be used for a high-concentration injection is obtained. After cell spheres are formed through 3D processing, the cell spheres are cultured in normal saline under a certain condition, the cell spheres can secrete a large number of secretory factors beneficial to cell survival under the condition, cells are removed after culture is conducted for a certain time, only secretory products of the cells are reserved, the culture supernatant is used in the conventional cell transportation process, and the cell survival rate is increased. The protection of the cells in the transportation process is facilitated. The preservation time can be prolonged, and the cell viability