Separation method and application of oroxylum indicum seed iron-promoting endophytic fungi

The invention discloses a separation method and application of iron-promoting endophytic fungi of oroxylum indicum seeds. The separation method mainly comprises the following steps: S1, seed pretreatment; s2, separating endophytic fungi; s3, identifying the ITS sequence of the strain; s4, determinin...

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Hauptverfasser:	ZHANG FENGRUI, FAN LIANGYU, LI JIAHUI, WANG KUN, XU HUITING, YI SONGHENG, YANG FAN, DING MUXI, LI WANLING, SHEN HONGQUAN, YANG YINXIN, SUN ZHENGHAI, LI JIAOZHEN, CHANG WANQI, LIU JIA, LI LIPING, XU JUANJUAN, ZHOU SHIJIA, MEN YUCHEN, CAI QIHANG, LI WEI, FANG JIARU, JIANG SHUJUN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPSOR SEAWEED CULTURE MEDIA ENZYMOLOGY FERTILISING FISHING FORESTRY HORTICULTURE HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PLANTING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SOWING SPIRITS TRAPPING VINEGAR WATERING WINE
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Beschreibung
Zusammenfassung:	The invention discloses a separation method and application of iron-promoting endophytic fungi of oroxylum indicum seeds. The separation method mainly comprises the following steps: S1, seed pretreatment; s2, separating endophytic fungi; s3, identifying the ITS sequence of the strain; s4, determining the activity of the siderophore of the strain; the iron-promoting endophytic fungi Penicilliumochrochloron which is obtained through separation and has the strongest iron-carrying capacity is inoculated to soybean seedlings, and experiments prove that the iron-promoting endophytic fungi separated from oroxylum indicum seeds have an obvious promoting effect on iron transfer and absorption of soybeans; the method is of great significance to research on promotion of plant iron absorption by the endophyte. 本发明公开了一种木蝴蝶种子促铁内生真菌的分离方法及其应用，其中分离方法主要包括：S1：种子预处理；S2：内生真菌分离；S3：菌株ITS序列鉴定；S4：菌株产铁载体活性测定；将分离得出的载铁能力最强的促铁内生真菌Penicilliumochrochloron接种至黄豆幼苗，经实验验证木蝴蝶种子中分离的促铁内生真菌对黄豆对铁的转运吸收具有明显的促进作用；对研究内生菌促进植物铁吸收研究具有重要的意义。