Siniperca chuatsi CRISPR/Cas9n gene editing method

The invention provides a siniperca chuatsi CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9n gene editing method, which comprises the following steps of: mixing Cas9n protein and target gene gRNA (guide ribonucleic acid), injecting the mixture into an ovum to be fertilized by utilizing a micro-injection mode, mixing 0.1 mg/mu L of Cas9n protein and 0.3 mg/mu L of gRNA, introducing the mixture into the ovum to be fertilized of the siniperca chuatsi by adopting a micro-injection method, and artificially inseminating to obtain a mutant embryo, after incubation, a siniperca chuatsi embryo with the target gene mutation rate as high as 85% can be obtained, so that a siniperca chuatsi target gene precise editing technology is established. The siniperca chuatsi embryo with the mutated target gene can be obtained by screening through a PAGE method. The technology can be used for researching the functions of the mandarin fish gene, and can also be used for accurately modifying the endogenous targ