Method for inducing colletotrichum truncatum to generate conidia

The invention discloses a method for inducing colletotrichum truncatum to generate conidia, which comprises the following specific steps: culturing colletotrichum truncatum on an improved PDA (potato dextrose agar) culture medium added with 60-90ppm of streptomycin sulfate for 6 days at the temperature of 25-28 DEG C, smashing and breaking hyphae by using a coating rod, and continuously culturing for 3 days. According to the method disclosed by the invention, through improved sectional culture, the spore production culture time of colletotrichum truncatum is shortened to 9 days from traditional 12 days, the culture time is reduced by 1/3, and the spore yield is increased to 1.0 * 10 < 8 > cfu/254 m < 2 > from 1.0 * 10 < 6 > cfu/254 m < 2 > and is increased by 100 times. 本发明公开了一种诱导平头炭疽菌产生分生孢子的方法，具体步骤为：在25-28℃下，将平头炭疽菌在加入60-90ppm硫酸链霉素的改良PDA培养基上培养6d后，用涂布棒将菌丝捣断，再继续培养3d。本发明通过改良的分段式培养，平头炭疽菌产孢培养时间由传统的12d缩短到了9d，减少1/3的培养时间，孢子产量由1.0×106cfu/254m2提高到1.0×108cfu/254m2，提高了100倍。