Display substrate and driving method thereof, display panel and display device

The invention provides a display substrate which comprises a substrate and a plurality of voltage transmission lines, and one end of each voltage transmission line extends into a first driving area and is connected with a corresponding voltage supply terminal; each pixel unit group comprises a plurality of pixel units, and each pixel unit comprises a driving transistor and a reset and data write-in circuit; the reset and data write-in circuit is configured to control the reset voltage provided by the voltage transmission line to be written into the grid electrode of the driving transistor, and write the data voltage into the grid electrode of the driving transistor after the configured preset interval duration from stopping writing the reset voltage into the grid electrode of the driving transistor; for any two adjacent pixel unit groups, the preset interval duration configured by the pixel units in the pixel unit group, close to the first driving area, in the two adjacent pixel unit groups is larger than the