Method for improving production efficiency of buffalo embryos in summer
The invention discloses a method for improving summer buffalo embryo production efficiency, and belongs to the technical field of buffalo in-vitro embryo development. According to the method, on the basis of conventional culture of buffalo oocytes, lithium chloride and melatonin are added into an in...
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| creator | ZHENG HAIYING SHANG JIANGHUA YANG CHUNYAN |
| description | The invention discloses a method for improving summer buffalo embryo production efficiency, and belongs to the technical field of buffalo in-vitro embryo development. According to the method, on the basis of conventional culture of buffalo oocytes, lithium chloride and melatonin are added into an in-vitro maturation culture solution for co-culture of the buffalo oocytes, and melatonin continues to be added for culture in the embryo culture stage. According to the method, in-vitro production efficiency of buffalo embryos in summer and winter and melatonin in buffalo bodies are researched and compared, the in-vitro production efficiency of the buffalo embryos in summer is obviously lower than that of buffalo embryos in winter, the concentration of melatonin in buffalo blood in summer is obviously lower than that of buffalo in winter, and it is presumed that melatonin can affect buffalo follicular development; further, oocyte maturation and embryonic development are influenced. According to the technical scheme, |
| format | Patent |
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According to the method, on the basis of conventional culture of buffalo oocytes, lithium chloride and melatonin are added into an in-vitro maturation culture solution for co-culture of the buffalo oocytes, and melatonin continues to be added for culture in the embryo culture stage. According to the method, in-vitro production efficiency of buffalo embryos in summer and winter and melatonin in buffalo bodies are researched and compared, the in-vitro production efficiency of the buffalo embryos in summer is obviously lower than that of buffalo embryos in winter, the concentration of melatonin in buffalo blood in summer is obviously lower than that of buffalo in winter, and it is presumed that melatonin can affect buffalo follicular development; further, oocyte maturation and embryonic development are influenced. According to the technical scheme,</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230117&DB=EPODOC&CC=CN&NR=115612659A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76419</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230117&DB=EPODOC&CC=CN&NR=115612659A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ZHENG HAIYING</creatorcontrib><creatorcontrib>SHANG JIANGHUA</creatorcontrib><creatorcontrib>YANG CHUNYAN</creatorcontrib><title>Method for improving production efficiency of buffalo embryos in summer</title><description>The invention discloses a method for improving summer buffalo embryo production efficiency, and belongs to the technical field of buffalo in-vitro embryo development. According to the method, on the basis of conventional culture of buffalo oocytes, lithium chloride and melatonin are added into an in-vitro maturation culture solution for co-culture of the buffalo oocytes, and melatonin continues to be added for culture in the embryo culture stage. According to the method, in-vitro production efficiency of buffalo embryos in summer and winter and melatonin in buffalo bodies are researched and compared, the in-vitro production efficiency of the buffalo embryos in summer is obviously lower than that of buffalo embryos in winter, the concentration of melatonin in buffalo blood in summer is obviously lower than that of buffalo in winter, and it is presumed that melatonin can affect buffalo follicular development; further, oocyte maturation and embryonic development are influenced. 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According to the method, on the basis of conventional culture of buffalo oocytes, lithium chloride and melatonin are added into an in-vitro maturation culture solution for co-culture of the buffalo oocytes, and melatonin continues to be added for culture in the embryo culture stage. According to the method, in-vitro production efficiency of buffalo embryos in summer and winter and melatonin in buffalo bodies are researched and compared, the in-vitro production efficiency of the buffalo embryos in summer is obviously lower than that of buffalo embryos in winter, the concentration of melatonin in buffalo blood in summer is obviously lower than that of buffalo in winter, and it is presumed that melatonin can affect buffalo follicular development; further, oocyte maturation and embryonic development are influenced. According to the technical scheme,</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Method for improving production efficiency of buffalo embryos in summer |
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