Method for improving production efficiency of buffalo embryos in summer

The invention discloses a method for improving summer buffalo embryo production efficiency, and belongs to the technical field of buffalo in-vitro embryo development. According to the method, on the basis of conventional culture of buffalo oocytes, lithium chloride and melatonin are added into an in-vitro maturation culture solution for co-culture of the buffalo oocytes, and melatonin continues to be added for culture in the embryo culture stage. According to the method, in-vitro production efficiency of buffalo embryos in summer and winter and melatonin in buffalo bodies are researched and compared, the in-vitro production efficiency of the buffalo embryos in summer is obviously lower than that of buffalo embryos in winter, the concentration of melatonin in buffalo blood in summer is obviously lower than that of buffalo in winter, and it is presumed that melatonin can affect buffalo follicular development; further, oocyte maturation and embryonic development are influenced. According to the technical scheme,