Pichia pastoris, preparation method thereof and application of pichia pastoris in catalyzing sucrose to produce fructo-oligosaccharide

The invention discloses pichia pastoris. Protein shown as SEQ ID No.7 is expressed on the cell surface of the pichia pastoris. The invention also discloses application of the catalyst in catalyzing sucrose to produce fructo-oligosaccharide, and a preparation method of the catalyst. The glucose oxidase-fructosyl transferase fusion enzyme is displayed on the surface of the pichia pastoris, whole cells of the pichia pastoris are used for catalyzing and producing the fructo-oligosaccharide, the inhibition effect of glucose on the reaction is eliminated during production, and the conversion rate of the fructo-oligosaccharide can be effectively improved. In addition, the invention also finds that the pichia pastoris of which the surface displays the glucose oxidase-fructosyl transferase fusion enzyme has higher enzyme activity and higher conversion rate of fructo-oligosaccharide when being used for catalytically producing the fructo-oligosaccharide than the pichia pastoris of the fructosyl transferase-glucose oxida