Hybridoma cell secreting anti-DNA polymerase monoclonal antibody, monoclonal antibody and application of monoclonal antibody

The invention discloses a hybridoma cell capable of secreting an anti-DNA polymerase monoclonal antibody, the monoclonal antibody and application of the monoclonal antibody, and relates to the technical field of preparation of hot start enzyme antibodies. The monoclonal antibody secreted by the hybridoma cell provided by the invention can completely close the activity of DNA polymerase. Compared with the existing hot start enzyme, the DNA polymerase modified by the monoclonal antibody has better stability, and the normal-temperature preservation time of the hot start enzyme is remarkably prolonged. And the monoclonal antibody secreted by the hybridoma cell has no nuclease activity residue. In addition, the monoclonal antibody obtained through screening can keep a good low-temperature sealing effect and a good high-temperature polymerization activity release effect on various DNA polymerases, and has good universality. The monoclonal antibody provided by the invention has the advantages of small dosage and goo