Trace protein quantitative detection reagent and detection method

The invention provides a trace protein quantitative detection reagent. The trace protein quantitative detection reagent comprises a DNA fragment marked by an affinity molecule A and an affinity molecule B, the affinity molecule B can mark a protein to be detected, and the affinity molecule A and the...

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Bibliographische Detailangaben
Hauptverfasser:	ZHANG XIAO, YANG XINGYU, CAO JUNFENG, XIA QINGJIE, SHENG ZIYI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a trace protein quantitative detection reagent. The trace protein quantitative detection reagent comprises a DNA fragment marked by an affinity molecule A and an affinity molecule B, the affinity molecule B can mark a protein to be detected, and the affinity molecule A and the affinity molecule B can be specifically combined with each other; the sequence of the DNA fragment is as shown in SEQ ID NO. 1. The invention also provides a method for quantitatively detecting the trace protein, the affinity molecule A is used for marking the to-be-detected protein, the affinity molecule B is used for marking the artificially synthesized DNA fragment, the characteristic that the affinity molecule A and the affinity molecule B can be specifically combined with each other is utilized, the DNA fragment is detected by a quantitative PCR technology, and the amount of the to-be-detected protein is reflected through the relative amount of DNA. The method is quick, simple and convenient to operate, and c