New coronavirus antigen detection method based on copper metal organic framework nano enzyme laccase, material and application

The invention provides a novel coronavirus antigen detection method based on copper metal organic framework nano enzyme laccase, a material and application, and the method mainly comprises the following steps: incubating a novel coronavirus N protein antibody, a bovine serum albumin solution, a novel coronavirus antigen solution and an Ab2 biological probe in sequence by using a 96-well plate, and detecting 2, 3, 4, 5, 6-tetramethyl-4-piperidine-1-one by using the biological probe. The ELISA biological immunosensor for detecting the novel coronavirus is prepared through a catalytic chromogenic reaction of 2, 4-dichlorophenol and 4-aminoantipyrine. The biosensor has the advantages of simple equipment, no need of professional operation, low cost, high sensitivity, visual detection, wide detection range, low detection limit and the like. The method has important scientific significance and clinical application value for detection of novel coronavirus. 本发明提供了基于铜金属有机框架纳米酶漆酶的新冠病毒抗原检测方法、材料及应用，其方法主要是使用96孔板依次将新型冠状病毒N蛋