Unsaturated iron binding force determination kit and application thereof

The invention discloses an unsaturated iron binding force determination kit and application thereof.The unsaturated iron binding force determination kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a BICINE buffer solution, ferric salt and a first preservative, the reagent R2 comprises a KCl-HCl buffer solution, a reducing agent, a color developing agent and a second preservative, the reagent R1 adopts an N, N-dihydroxyethylglycine (BININE buffer solution, the stability of the reagent R1 is improved, no additional chelating agent needs to be introduced, and the detection sensitivity is improved. Good stability of the kit can be maintained without adding a stabilizing agent; the reagent R1 adopts a KCl-HCl biological buffer solution, the reagent R2 adopts a KCl-HCl biological buffer solution, the buffer solution is not strong in buffering capacity and does not influence the alkaline environment after the reagent R1 and the reagent R2 are mixed, and KCl can maintain electrolyte balance of a