Method for introducing fluorescent dye into lily bulb under in-vitro condition

The invention provides a method for introducing a fluorescent dye into a lily bulb under an in-vitro condition and a fluorescent tracing method for researching an assimilation transportation direction and path of an in-vitro lily plant. The method for introducing the fluorescent dye into the lily bulb under the in-vitro condition comprises the following steps: step S10, taking an outer scale of the in-vitro lily bulb subjected to metabolic culture in a bud inducing culture medium as a to-be-treated scale, and forming a wedge-shaped notch in the middle of a far-axis end of the to-be-treated scale under an aseptic condition, the wedge-shaped notch penetrating through a vascular bundle; s20, a water absorption material is stuffed into the wedge-shaped notch, and fluorescent dye is injected into the water absorption material; and step S30, after the injection is finished, carrying out sealing and light shielding treatment on the wedge-shaped notch. The introduction of the fluorescent dye does not need special equ