Method for improving lentivirus transfection efficiency

The invention belongs to the field of biological medicines, and particularly relates to a technology for improving the transfection efficiency of chimeric antigen recipient cells. In particular to a technology for improving the transfection efficiency of chimeric antigen recipient cells by using the...

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Bibliographische Detailangaben
Hauptverfasser: YANG ZHI, HONG JUAN, JIAO LIURONG, XU YANMIN, SHEN JUNJIE, HUANG YUKANG, QI YANAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the field of biological medicines, and particularly relates to a technology for improving the transfection efficiency of chimeric antigen recipient cells. In particular to a technology for improving the transfection efficiency of chimeric antigen recipient cells by using the composition, the composition comprises DMSO and DEAE, the mass ratio of DMSO to DEAE is (1: 4)-(11: 4), and compared with independent use of each component, the composition has the advantages of simplicity in operation, time and cost saving and higher transfection efficiency. 本技术属于生物医药领域,具体涉及嵌合抗原受体细胞提升转染效率的提升技术。具体为用组合物提升嵌合抗原受体细胞提升转染效率的技术,所述组合物中包括DMSO和DEAE,两者的质量比为1:4-11:4,本组合物使用相对于各成分单独使用具有操作简单、节约时间成本并且转染效率更高。