Cultivation, identification and application of rice microfilament depolymerization factor ADF6 transgenic plant

The invention belongs to the technical field of molecular plant breeding and gene splicing and DNA (deoxyribonucleic acid) recombination, and provides a recombinant vector comprising a rice OsADF6 gene, a transformed cell, and cultivation, identification and application of arabidopsis thaliana trans...

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Bibliographische Detailangaben
Hauptverfasser:	WANG LINA, CHENG JIANING, WANG CHE, BI SHUANGTIAN, WANG JINSHU, CHENG XIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention belongs to the technical field of molecular plant breeding and gene splicing and DNA (deoxyribonucleic acid) recombination, and provides a recombinant vector comprising a rice OsADF6 gene, a transformed cell, and cultivation, identification and application of arabidopsis thaliana transfected with the OsADF6 gene. The cultivation method of the OsADF6 transgenic arabidopsis thaliana comprises the following steps: cloning the rice OsADF6 gene, constructing a plant expression vector PCAMBIA1300-221-OsADF6, and transforming the arabidopsis thaliana by an agrobacterium GV3101 dip dyeing method. And carrying out PCR (Polymerase Chain Reaction) detection and fluorescent quantitative RT-PCR detection on the transformed plant to verify that the rice OsADF6 gene is integrated into an arabidopsis plant genome and is normally transcribed and expressed. The transgenic plant is detected, the drought resistance of the transgenic plant is obviously improved, and technical support is provided for further related