Development method of HEK293 suspension cell line for viral vector production

The invention relates to a development method of a suspension HEK293 cell line for viral vector production. The method comprises the following steps: cloning and screening HEK293 adherent cells by a limited dilution method to obtain 180-250 cell clones; evaluating the growth rate and virus vector transfection efficiency of the cell clones obtained by cloning and screening, and screening 15-25 groups of cell clones to be subjected to enlarged culture; respectively carrying out amplification culture on each group of cell clones to be subjected to amplification culture, and screening according to the growth rate and the virus vector transfection efficiency to obtain 5-13 groups of preferable clone cells for suspension domestication; respectively carrying out suspension domestication on each group of preferable clone cells; and respectively taking a part of each group of cells subjected to suspension domestication, and screening according to virus vector transfection efficiency to prepare the HEK293 cells suitabl