Living cell DNA marker signal amplification method based on CRISPR/dCas9 system and oligonucleotide probe

The invention discloses a living cell DNA marker signal amplification method based on a CRISPR/dCas9 system and an oligonucleotide probe, an existing CRISPR/MB platform is modified by increasing the number of organic dyes carried by a single sgRNA, a TS sequence which is repeatedly connected in series is inserted into a step-loop 2 region of the sgRNA by using a molecular synthesis technology, the TS sequence corresponds to multiple groups of donor MB and receptor MB capable of generating FRET, and the number of the organic dyes carried by the single sgRNA is increased. Therefore, effective signal intensity amplification is realized while the number of dCas9-sgRNA complexes for marking a single DNA site is not increased, and a universal signal amplification method is provided for a living cell DNA marking method based on a CRISPR/dCas9 system and an oligonucleotide probe. 本发明公开了一种基于CRISPR/dCas9系统与寡核苷酸探针的活细胞DNA标记信号放大方法，通过增加单个sgRNA所携带的有机染料数目对现有的CRISPR/MB平台进行改造，运用分子合成技术在sgRNA的stem-loop 2区域插入串联重复多次的TS序列，对应多组能够发生F