Detection kit for detecting Japanese B encephalitis virus nucleic acid based on gene editing technology and application

The invention discloses a detection kit for detecting Japanese B encephalitis virus nucleic acid based on a gene editing technology and application of the detection kit. CrRNA with high activity and a pair of amplification primers with high sensitivity and high specificity are obtained through screening, and a CRISPR-Cas12a detection system is combined with a double-labeled fluorescent probe (ROX); and the amplification reaction can be completed in a heating type vacuum cup. Finally, the detection result can be directly visible by naked eyes or fluorescent light, the detection sensitivity for Japanese B encephalitis virus nucleic acid is 9 copies/microliter, and other viruses susceptible to pigs, such as ASFV, PRRSV, PDCoV and TGEV, can be specifically distinguished. The method is convenient to operate and simple in equipment, has the advantages of low cost, time saving, high efficiency, high specificity, high sensitivity and the like, can be widely applied to various big pig breeding enterprises, improves th