Cable-shaped decellularized nerve scaffold as well as preparation method and application thereof

The invention relates to the technical field of nerve regeneration biology, in particular to a cable-shaped decellularized nerve scaffold as well as a preparation method and application thereof. The method specifically comprises the following steps: (1) mixing nerves with washing liquid, and shaking; (2) washing the nerves obtained in the step (1) with a 4 * PBS buffer solution, a 2 * PBS buffer solution and a 1 * PBS buffer solution in sequence; (3) mixing the nerves obtained in the step (2) with DNaseI, washing with a 1 * PBS buffer solution, and storing with the 1 * PBS buffer solution; and (4) performing radiation sterilization on the nerves obtained in the step (3). Camptothecin is used for inducing apoptosis of cells in peripheral nerves, then hypertonic phosphate buffer saline and deoxyribonuclease are used for removing cell fragments and DNA fragments in the peripheral nerves, a complete extracellular matrix structure is reserved, and immunogenic tolerance is shown after the extracellular matrix is im