Biological agent immunocompetent substance excavation and evaluation method based on cytokine determination

The method comprises the following steps: (1) preparing a test solution, (2) preparing a reference solution: dissolving a reference chain with sterile water and diluting the reference chain to obtain the reference solution, and (3) culturing macrophages: taking unpolarized macrophages, culturing and planking the unpolarized macrophages to obtain the biological preparation immunocompetent substance excavation and evaluation method based on cytokine determination, and obtaining the biological preparation immunocompetent substance excavation and evaluation method based on cytokine determination. (4) co-incubating the medicine and the cells; (5) detecting cell factors; and (6) functional index determination: calculating the functional index (IFC) of the test solution for stimulating the macrophages to release cytokines (CF) according to a formula according to the concentration of the test solution for stimulating the macrophages to release the TNF-alpha, which is measured in the step (5), of the test solution for