METHOD FOR PRODUCING CELL SUSPENSION AND METHOD FOR PRODUCING ADHESIVE CELLS

One embodiment of the present invention relates to a method for producing a cell suspension, the method comprising the following (A), (B) and (C): (A) a step for culturing adhered cells in a cell suspension containing the adhered cells, a microcarrier and a culture medium and having a volume of 0.3...

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Bibliographische Detailangaben
Hauptverfasser:	OKANO, SADAHIRO, SUZUKI YUGO, NAKAJIMA KATSUHIKO, TADA YASUHIKO, TAKAHASHI RYOSUKE, CHEN SHANGWU, NAKAGAWA FUMIKO, SATO YUJI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	One embodiment of the present invention relates to a method for producing a cell suspension, the method comprising the following (A), (B) and (C): (A) a step for culturing adhered cells in a cell suspension containing the adhered cells, a microcarrier and a culture medium and having a volume of 0.3 L or more; (B) a step for culturing the adhered cells in a cell suspension containing the adhered cells obtained in (A), a microcarrier, and a culture medium and having a volume of 5 L or more; and (C) a step for culturing the adhered cells in a cell suspension containing the adhered cells obtained in (B), a microcarrier, and a culture medium and having a volume of 10 L or more. 本发明的一个实施方式涉及一种细胞悬液的制造方法，其包括下述(A)、(B)和(C)，(A)在含有黏附细胞、微载体和培养基，并且体积为0.3L以上的细胞悬液中培养所述黏附细胞的工序；(B)在含有经过所述(A)得到的黏附细胞、微载体和培养基，并且体积为5L以上的细胞悬液中培养所述黏附细胞的工序；以及(C)在含有经过所述(B)得到的黏附细胞、微载体和培养基，并且体积为10L以上的细胞悬液中培养所述黏附细胞的工序。