Protein-coated latex microsphere reagent and coating method

The invention provides a protein-coated latex microsphere reagent and a coating method. The coating method comprises the following steps: carrying out an activation reaction on latex microspheres by adopting a mixed activator to obtain activated microspheres, diluting the activated microspheres and the protein by using a coupling buffer solution to respectively obtain diluted activated microspheres and diluted protein; carrying out coupling reaction on the diluted activated microspheres and diluted protein to obtain a coupling compound; sequentially carrying out sealing and storage treatment on the coupled compound to obtain a protein-coated latex microsphere solution; wherein the coupling buffer solution is selected from any one of a borax buffer solution, a phosphate buffer solution, an HEPES buffer solution and an MOPS buffer solution; the mixed activating agent is a mixed solution of EDC and Sulfo-NHS. The method solves the problem that the latex microsphere coupling antibody or antigen related reagent pr