Method for determining levofloxacin related substances by liquid chromatography-mass spectrometry

The invention discloses a method for measuring levofloxacin related substances through liquid chromatography-mass spectrometry, belongs to the technical field of pharmaceutical analysis, and solves the problems that in the prior art, the detection chromatographic peak shape of the related substances is poor, the response value is low, and the detection sensitivity requirement cannot be met. The liquid phase conditions of the method are as follows: octadecylsilane chemically bonded silica is used as a filler, an ammonium formate-formic acid aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and gradient elution is performed; the mass spectrum conditions are as follows: a mass spectrum detector is adopted, an electrospray ionization positive ion mode is adopted, the mass-to-charge ratio is 392, and the related substance is levofloxacin ethylene glyoxal. According to the method, the content of the related substance levofloxacin to ethylene glyoxal is measured by adopting a li