Method for mass production of protein of interest from plants

The present invention relates to a method for mass production of a target protein from a plant, and more particularly, to an expression kit construct for high expression of a target protein and an expression kit construct for high expression of a gene silencing suppressor p38, and to a method for ma...

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Bibliographische Detailangaben
Hauptverfasser: KANG HYANG JU, MUSAMIR, SELVAN, THANGARASU, HUANG RENHUAN, LEE JUN-HO, SONG SHIJIAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The present invention relates to a method for mass production of a target protein from a plant, and more particularly, to an expression kit construct for high expression of a target protein and an expression kit construct for high expression of a gene silencing suppressor p38, and to a method for mass production of a target protein from a plant, the present invention relates to a method for producing a protein of interest in a plant leaf at a high level by culturing a single Agrobacterium tumefaciens, and then preparing a binary vector for transient expression in a plant cell, which simultaneously has the above-mentioned construct (binary vector), which is cultured using a single Agrobacterium tumefaciens, by Agrobacterium tumefaciens-mediated transformation. 本发明涉及从植物中大量生产目标蛋白质的方法,具体地,涉及制备目标蛋白质(target gene)的高表达用表达试剂盒构建体及基因沉寂抑制子(gene silencing suppressor)p38的高表达用表达试剂盒构建体,然后制备同时具有上述构建体的在植物细胞中的瞬时表达用(transient expression)双元载体(双元载体),所述双元载体利用单一农杆菌培养,利用农杆菌-介导转化,在植物叶子中以高水平生产目标蛋白质的方法。