Method for mass production of protein of interest from plants
The present invention relates to a method for mass production of a target protein from a plant, and more particularly, to an expression kit construct for high expression of a target protein and an expression kit construct for high expression of a gene silencing suppressor p38, and to a method for ma...
Gespeichert in:
Hauptverfasser: | , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The present invention relates to a method for mass production of a target protein from a plant, and more particularly, to an expression kit construct for high expression of a target protein and an expression kit construct for high expression of a gene silencing suppressor p38, and to a method for mass production of a target protein from a plant, the present invention relates to a method for producing a protein of interest in a plant leaf at a high level by culturing a single Agrobacterium tumefaciens, and then preparing a binary vector for transient expression in a plant cell, which simultaneously has the above-mentioned construct (binary vector), which is cultured using a single Agrobacterium tumefaciens, by Agrobacterium tumefaciens-mediated transformation.
本发明涉及从植物中大量生产目标蛋白质的方法,具体地,涉及制备目标蛋白质(target gene)的高表达用表达试剂盒构建体及基因沉寂抑制子(gene silencing suppressor)p38的高表达用表达试剂盒构建体,然后制备同时具有上述构建体的在植物细胞中的瞬时表达用(transient expression)双元载体(双元载体),所述双元载体利用单一农杆菌培养,利用农杆菌-介导转化,在植物叶子中以高水平生产目标蛋白质的方法。 |
---|