Compositions and methods for nucleic acid amplification

Described herein are methods for amplifying and detecting specific nucleic acids in a nucleic acid sample. The method can include amplifying a target nucleic acid in the presence of a fluorescent probe and a probe complement and detecting the target nucleic acid. Also described herein are probe comp...

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Hauptverfasser:	SHEN EILEEN YI LIN, FAN HAI MAN CHRISTINA
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Described herein are methods for amplifying and detecting specific nucleic acids in a nucleic acid sample. The method can include amplifying a target nucleic acid in the presence of a fluorescent probe and a probe complement and detecting the target nucleic acid. Also described herein are probe complements for detecting specific nucleic acid sequences with reduced background fluorescence. 本文描述了用于扩增和检测核酸样品中特定核酸的方法。该方法可包括在荧光探针和探针补体存在下扩增靶核酸并检测该靶核酸。本文还描述了用于检测背景荧光降低的特定核酸序列的探针补体。