Method for identifying aegilops tauschii derived germplasm fragments by utilizing wheat crop D genome exon capture sequencing chip
The invention relates to a method for identifying aegilops tauschii derived germplasm fragments by using a wheat crop D genome exon capture sequencing chip. Comprising the following steps: carrying out capture sequencing on a derived introgression line material and parents of the derived introgressi...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a method for identifying aegilops tauschii derived germplasm fragments by using a wheat crop D genome exon capture sequencing chip. Comprising the following steps: carrying out capture sequencing on a derived introgression line material and parents of the derived introgression line material by using a wheat D genome generic gene exon capture sequencing chip; comparing the derived introgression line material and the captured sequencing data of the parents of the derived introgression line material to a wheat reference genome to obtain a derived introgression line material difference site file and a parent difference site file; filtering the parental difference site file and the introgression line material difference site file, and retaining the intersection of the two difference site files; counting the exogenous infiltration proportion of the derivative introgression line material in the window by using the intersection file and the parent difference site file; and drawing a point dia |
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