Method for identifying aegilops tauschii derived germplasm fragments by utilizing wheat crop D genome exon capture sequencing chip

The invention relates to a method for identifying aegilops tauschii derived germplasm fragments by using a wheat crop D genome exon capture sequencing chip. Comprising the following steps: carrying out capture sequencing on a derived introgression line material and parents of the derived introgressi...

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Hauptverfasser: SONG CHUNPENG, LI ZHENG, LI CHENGLIN, LIU WENJUAN, LIU YIFAN, ZHU LELE, NIE FANG, LI HAO, LI CAN, GUO TUTU, LIU XUQIN, ZHU YU, FAN RUIXIAO, BU MENGCHEN, YANG YINGYING, ZHOU YUN, LI YUANYUAN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a method for identifying aegilops tauschii derived germplasm fragments by using a wheat crop D genome exon capture sequencing chip. Comprising the following steps: carrying out capture sequencing on a derived introgression line material and parents of the derived introgression line material by using a wheat D genome generic gene exon capture sequencing chip; comparing the derived introgression line material and the captured sequencing data of the parents of the derived introgression line material to a wheat reference genome to obtain a derived introgression line material difference site file and a parent difference site file; filtering the parental difference site file and the introgression line material difference site file, and retaining the intersection of the two difference site files; counting the exogenous infiltration proportion of the derivative introgression line material in the window by using the intersection file and the parent difference site file; and drawing a point dia