Plant mitochondrial genome multi-configuration assembly method based on third-generation whole genome sequencing data

The invention discloses a plant mitochondrial genome multi-configuration assembly method based on third-generation whole genome sequencing data, and belongs to the technical field of biological information. According to the method, a set of plant mitochondrial genome multi-configuration assembly process suitable for third-generation full-platform (PacBio/Nanopore/HiFi) sequencing data is constructed by utilizing the characteristic that NewBler assembly software can retain possible connection among all Contigs, copy number differences among mitochondria, chloroplast and cell nucleus sequences in third-generation WGS data and the advantages that third-generation sequencing reads can cross repetitive sequences. According to the process, when multiple branches caused by repetitive sequences are encountered in the process of assembling the mitochondrial genome, all possible connections can be reserved, and extension continues until a closed loop is formed, so that all possible configurations of the mitochondrial g