Culture medium and method for inducing iPSC (induced pluripotent stem cell) differentiation to obtain macrophages and application of culture medium and method

The invention relates to a culture medium and method for inducing iPSC differentiation to obtain macrophages and application of the culture medium and method, and relates to the technical field of biological medicine. The first-stage culture medium is an E8 complete culture medium containing a ROCK pathway inhibitor and polyvinyl alcohol, the second-stage culture medium is an E8 complete culture medium containing a GSK-3beta inhibitor, the third-stage culture medium comprises an M1 culture medium and an M2 culture medium, the fourth-stage culture medium is an M3 culture medium, and the fifth-stage culture medium is an M4 culture medium. And the sixth stage is an M5 culture medium. By optimizing the culture mode and culture conditions, the yield of the macrophages is increased, the culture cost is reduced, about 18.80 thousand macrophages can be obtained from one iPSC, and the obtained macrophages have a good phagocytic function. 本发明涉及一种诱导iPSC分化获得巨噬细胞的培养基、方法及其应用，涉及生物医药技术领域，包括第一阶段培养基至第六阶段培养基；所述第一阶段培养基为含有ROCK通路抑