Cell digestive juice and method for isolated culture of oral mucosa epithelial cells by using cell digestive juice

The invention relates to the technical field of cell isolated culture, and particularly discloses a cell digestive juice and a method for isolated culture of oral mucosa epithelial cells by using the cell digestive juice. The invention discloses a cell digestive juice, which comprises the following components: 4-8 g/L protease; the concentration of adenosine disodium triphosphate is 0.2-0.6 g/L; and 0.09 to 0.15 g/L of an enzyme activator. The invention further discloses application of the cell digestive juice in the field of cell separation and culture. When the cell digestive juice is used for carrying out isolated culture on oral mucosa epithelial cells, the digestion time can be effectively shortened, and meanwhile, a large number of cells with high survival rate can be obtained. 本申请涉及细胞分离培养的技术领域，具体公开了一种细胞消化液以及利用该细胞消化液对口腔黏膜上皮细胞分离培养的方法。本申请公开了一种细胞消化液，包括以下浓度的组分：蛋白酶4-8g/L；三磷酸腺苷二钠0.2-0.6g/L；酶激活剂0.09-0.15g/L。本申请还公开了上述细胞消化液在细胞分离培养领域中的应用。利用该细胞消化液对口腔黏膜上皮细胞进行分离培养时，能够有效缩短消化时间，同时能够获得数量多且存活率高的细胞。