Preparation method of 3D printing osteochondral scaffold with three-layer structure

The invention relates to the technical field of biomedical tissue engineering, in particular to a preparation method of a three-layer-structure 3D printing osteochondral scaffold, the multilayer structure of natural osteochondral is simplified, a three-layer-structure simulation in-situ osteochondral structure is constructed, meanwhile, different components are used for forming a structural layer, and the three-layer-structure 3D printing osteochondral scaffold is prepared. According to the present invention, the characteristic of mechanical strength increasing is achieved, the growth fusion with the natural osteochondral tissue after implantation is easily achieved through the bionic structure, and further, the 3D printing porous structure is adopted in the whole scaffold so as to easily enter the nutritional components in the environment, easily migrate somatic cells to the scaffold, and improve the implantation effect of the bionic scaffold; the technical problem that an existing osteochondral repair techn