Preparation method of scallop male haploid embryo

The invention provides a preparation method of a scallop androgenetic haploid embryo, which comprises the following steps: carrying out backcross fertilization on an ovum of bay scallop and an ovum of an all-female individual in a first filial generation cultured by fertilization of sperms of argope...

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Hauptverfasser: DONG XUECHENG, ZHANG JUNHAO, LU WEI, XING QIANG, LIU ANCHENG, WANG WEN, YANG ZUJING, HOU XIUJIANG, HUANG XIAOTING, BAO ZHENMIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a preparation method of a scallop androgenetic haploid embryo, which comprises the following steps: carrying out backcross fertilization on an ovum of bay scallop and an ovum of an all-female individual in a first filial generation cultured by fertilization of sperms of argopecten purpuratus and sperms of bay scallop to obtain the androgenetic haploid embryo. The novel method for preparing the scallop androgenetic haploid embryo is created through spawning of the argopecten irradians diploid and natural fertilization of the argopecten irradians diploid and sperms of the argopecten irradians. According to the method for obtaining the scallop male haploid embryo, the ova of the argopecten irradians and the ova of all-female individuals in the first filial generation are fertilized and cultivated through sperms of the argopecten purpuratus are then subjected to backcross fertilization with the sperms of the argopecten irradians, and the method has the advantages of being natural in fertili