Fluorescence detection nanoprobe, preparation method and application

The invention discloses a preparation method of a fluorescence detection nanoprobe. The method comprises the following steps: synthesizing and preparing blue fluorescence carbon dots (BCD) through a hydrothermal method; synthesizing and preparing red fluorescent carbon dots (RCD) through a solvothermal method; and uniformly mixing the prepared BCD and RCD according to a certain proportion, co-incubating with Wotmann filter paper, and drying by a drying oven to obtain the fluorescence detection nano probe. The invention also discloses a fluorescence detection nano probe and application thereof. The method has the beneficial effects that the fluorescence detection performance is relatively good, and the detection method is simple, rapid and relatively high in sensitivity. 本发明公开了一种荧光检测纳米探针的制备方法，该方法包括如下步骤：通过水热法合成制备蓝色荧光碳点（BCD）；通过溶剂热法合成制备红色荧光碳点（RCD）；将制备的BCD和RCD以一定比例混匀，与沃特曼滤纸共孵育，经烘箱干燥后得到荧光检测纳米探针。本发明还公开了一种荧光检测纳米探针及其应用。本发明的有益效果在于：具有较好的荧光检测性能，检测方法简单、快捷，灵敏度较高。