High-degree-of-freedom microscopic image splicing and fusing method and system

The invention provides a high-degree-of-freedom microscopic image splicing and fusing method and system, relates to the technical field of image splicing and fusing, and aims to consider that a microscopic main image is determined and the final image ImgLast spliced into the microscopic main image is determined in the actual image splicing process. The method comprises the following steps: determining an accurate relative displacement between an image ImgLast and an image to be stitched in an overlapping region of other positions of the image to be stitched and the microscopic main image except the image ImgLast if the overlapping region is multi-region and polygonal overlapping, and guiding weight distribution value calculation when points in the overlapping region of the image to be stitched and the microscopic main image are fused; the weight distribution value is used as a reference, the gray value of an overlapped graph after each point of the to-be-spliced image at the same position is fused with the mi