Method for detecting aloein content in fresh aloe juice and application thereof

The invention relates to a method for detecting the content of aloesin in fresh aloe juice and application of the method. The detection method comprises the following steps: preparing a reference substance solution, preparing a test solution, and testing chromatographic conditions and system applicability. The detection method has the advantages that the peak appearance time of the aloesin is 3-4 minutes, the aloesin detection quantitation limit can reach 0.001 mg/g, the sensitivity is high, the reproducibility is good, the precision is high and the like, the detection standard of the aloe juice is improved, and the quality of the aloe medicine is effectively controlled, so that the application safety of the aloe medicine is ensured. 本发明涉及一种检测鲜芦荟汁中芦荟苦素含量的方法及其应用，其检测方法为包括对照品溶液的制备、供试品溶液的制备、色谱条件与系统适用性试验。该检测方法芦荟苦素出峰时间为3～4min，芦荟苦素检出定量限能达到0.001mg/g，灵敏度高，重现性好，精密度高等优点，提升了芦荟汁的检测标准，有效控制了芦荟药物质量，从而保证芦荟药材应用的安全性。