Method for detecting multiple steroid hormones in biological fluid
The invention relates to the field of G01N30/02, in particular to a method for detecting multiple steroid hormones in biological fluid. The invention discloses a method for detecting multiple steroid hormones in biological fluid. The method comprises the following steps: (1) preparing a standard sol...
Gespeichert in:
| Hauptverfasser: | , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| Zusammenfassung: | The invention relates to the field of G01N30/02, in particular to a method for detecting multiple steroid hormones in biological fluid. The invention discloses a method for detecting multiple steroid hormones in biological fluid. The method comprises the following steps: (1) preparing a standard solution and pre-treating a sample; preparing a standard solution of hormone metabolites of the isotope internal standard and a urine mixed solution of the isotope internal standard; respectively carrying out enzymolysis and solid-liquid extraction on the urine mixed solution of the isotope internal standard substance and the standard solution of the hormone metabolite of the isotope internal standard substance, and carrying out derivatization pretreatment after nitrogen blowing to respectively obtain a reference substance solution and a test substance solution; (2) carrying out gas chromatography-mass spectrometry detection; and (3) quantitative detection. The method for detecting various steroid hormones in biologic |
|---|