Method for researching influence of different processing methods on number of pueraria lobata chromosomes

The invention discloses a method for researching the influence of different treatment methods on the number of chromosomes of pueraria lobata, and the method for researching the influence of different treatment methods on the number of chromosomes of pueraria lobata comprises the following steps: S1, respectively carrying out mutagenesis treatment on pueraria lobata buds by using an EMS mutagenic agent, colchicine and the EMS mutagenic agent and colchicine; s2, planting pueraria buds subjected to different mutagenesis treatment in a field; s3, respectively taking kudzuvine root tips grown from the kudzuvine root buds subjected to different mutagenesis treatments, preparing slices, and determining the number of chromosomes; and S4, carrying out comparative analysis on the number of chromosomes in kudzuvine roots grown from the kudzuvine root buds subjected to different mutagenesis treatment. According to the invention, the theoretical basis is laid for mutation breeding, excellent breeding, development and uti