Semen cassiae fingerprint spectrum construction and detection method, basic identification construction method and application of semen cassiae fingerprint spectrum construction and detection method

The invention provides a semen cassiae fingerprint spectrum construction method which comprises the following steps: taking a red streptomycin-6-O-beta-D-gentiobioside reference substance and an aurantio-obtusin reference substance, and preparing a mixed reference substance solution; taking a semen cassiae sample, and preparing a test solution; respectively carrying out ultra-high performance liquid chromatography analysis on the mixed reference substance solution and the test solution, and constructing a fingerprint spectrum of the semen cassiae; wherein the conditions of the ultra-high performance liquid chromatography are as follows: a mobile phase A is a mixed solution of acetonitrile and methanol, and a mobile phase B is a phosphoric acid aqueous solution; gradient elution is adopted. The method has the advantages of being easy to operate, rich in map information, high in characteristic feature and good in reproducibility, and a comprehensive, effective and rapid evaluation method is provided for quality