Aquatic product freshness detection method and kit

The invention belongs to the technical field of aquatic product freshness detection, and discloses an aquatic product freshness detection method and a kit. According to the method, a nucleic acid degradation product hypoxanthine in the aquatic product is used as a detection marker, manganese dioxide...

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Hauptverfasser: CONG HAOJIE, WANG XIAOKUN, DING HAO, SHANG HEZHEN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention belongs to the technical field of aquatic product freshness detection, and discloses an aquatic product freshness detection method and a kit. According to the method, a nucleic acid degradation product hypoxanthine in the aquatic product is used as a detection marker, manganese dioxide is decomposed by using a product after an enzyme reaction, the etching reaction of the gold nanorods catalyzed by manganese dioxide is prevented, and the detection purpose of identifying the freshness of the aquatic product is achieved. The detection method and the kit provided by the invention have the advantages of short reaction time, high detection speed, strong specificity, high sensitivity, low detection limit, bright color output, naked eye visualization and the like, and are suitable for on-site real-time detection; especially for aquatic products in the initial decay stage, the detection method provided by the invention can be used for sensitively and accurately judging the initial freshness of the aquati