Method for extracting adenosine from Raffasia lauricola

The method comprises the following steps: activating a bacterial strain, performing primary culture, performing secondary culture, grinding thalli, extracting with ethyl acetate, concentrating to obtain a crude extract, performing reverse phase silica gel column chromatography on the crude extract,...

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Hauptverfasser:	LAI PENGYU, TAN ZHEN, LIU SEN, ZHU ZHIQIANG, QIU JUNZHI, REN GUANRU, CHEN JINHUI, PU HUILI, YANG CHENJIE, CHEN YUXI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The method comprises the following steps: activating a bacterial strain, performing primary culture, performing secondary culture, grinding thalli, extracting with ethyl acetate, concentrating to obtain a crude extract, performing reverse phase silica gel column chromatography on the crude extract, performing gradient elution with methanol and water, performing HPLC (High Performance Liquid Chromatography) preparation, and performing 1H NMR, 13C NMR, COSY, HMBC and HSQC spectrogram analysis to prove that adenosine is extracted from a metabolite of the bacterial strain for the first time. 本发明公开了一种从Raffaelea lauricola中提取腺苷的方法，包括：活化菌株，初级培养，次级培养，菌体研磨，乙酸乙酯萃取，浓缩得粗提物，将粗提物进行反相硅胶柱层析，以甲醇：水梯度洗脱，再通过HPLC制备，经1H NMR、13C NMR、COSY、HMBC、HSQC谱图分析证明腺苷首次在该菌种代谢物中提取到。