HPLC (High Performance Liquid Chromatography) detection method for liquiritigenin in Caragana changii

The invention discloses an HPLC (High Performance Liquid Chromatography) detection method for liquiritigenin in Caragana changii, which comprises the following steps: 1) preparation of a reference substance solution: taking a liquiritigenin reference substance, and adding methanol to dissolve the liquiritigenin reference substance to obtain the reference substance solution; 2) preparation of a test solution: taking a to-be-detected sample, adding methanol for extraction, filtering an extracting solution, and taking filtrate as the test solution; (3) respectively sucking the reference solution and the test solution and injecting into a high performance liquid chromatograph; chromatographic conditions are as follows: a chromatographic column takes octadecylsilane chemically bonded silica as a filler; and a mobile phase comprises 0.1% formic acid water and methanol in a volume ratio of 68: 32. Through a specific sample pretreatment method and chromatographic conditions, complete separation of the important compo