Living cell endoplasmic reticulum autophagy imaging analysis compound and preparation method thereof

The invention discloses a living cell endoplasmic reticulum autophagy imaging analysis compound and a preparation method thereof, the living cell endoplasmic reticulum autophagy imaging analysis compound is a fluorescent probe ER-proRed, the structural formula of the fluorescent probe is that under the action of FHR, green fluorescence is enriched and emitted in an endoplasmic reticulum; when endoplasmic reticulum autophagy occurs, the kit enters an acid lysosome along with the endoplasmic reticulum, and an acid-responsive strong red fluorescence signal is started, so that the analysis of the endoplasmic reticulum autophagy process is realized. 本发明公开了一种活细胞内质网自噬成像分析化合物及其制备方法，其为荧光探针ER-proRed，其结构式为在FHR的作用下，本发明在内质网内富集，发出绿色荧光；当发生内质网自噬时，本发明伴随着内质网进入酸性溶酶体，开启酸响应的强烈红色荧光信号，从而实现对内质网自噬过程的分析。