Piglet weaning stress immunodetection method based on biomass spectrum

The invention discloses a piglet weaning stress immunodetection method based on a biomass spectrum, which comprises the following steps: respectively detecting a test piglet serum sample and a control piglet serum sample by using a liquid chromatograph-mass spectrometer; the mass spectrum is corrected due to sample difference randomness and fluctuation interference of complex ion signals of various heavy chain polypeptides in immunoglobulin; further accurately obtaining a test polypeptide list and a control polypeptide list, and screening to obtain a characteristic immune polypeptide segment caused by the weaning stress condition; and establishing a stress response intensity change curve according to the characteristic immune polypeptide, and evaluating the immune function of the test piglet under the weaning stress condition, thereby achieving the purpose of analyzing the immune function of the piglet under the weaning stress condition. 本发明公开了一种基于生物质谱的仔猪断奶应激性免疫检测方法，利用液相色谱质谱联用仪分别检测试验仔猪血清样本和对照仔猪血清样本，对质谱进行校正由于样