HPLC (High Performance Liquid Chromatography) detection and separation method for celecoxib intermediate or/and impurity p-phenylhydrazine sulfonate

The invention discloses an HPLC (High Performance Liquid Chromatography) detection and separation method for a celecoxib intermediate or/and an impurity p-sulfoacid phenylhydrazine, belongs to the technical field of pharmaceutical analysis, and solves the problems that the impurity p-sulfoacid phenylhydrazine and the intermediate p-sulfonamide phenylhydrazine cannot be effectively separated, the operation is complicated and the sensitivity is not high in the prior art. Chromatographic conditions of the HPLC detection method and the separation method for the celecoxib intermediate or/and the impurity phenylhydrazine sulfonate are as follows: a hydrophilic chromatographic filler is used as a stationary phase, an ammonium formate solution is used as a mobile phase A, and acetonitrile is used as a mobile phase B for isocratic elution. In the detection method disclosed by the invention, the p-sulfonamide phenylhydrazine and the p-sulfonamide phenylhydrazine hydrochloride can be well separated, the sensitivity is g