Recombinant pancreatic cancer cell capable of being used for exosome tracing and application of recombinant pancreatic cancer cell
The invention provides a recombinant pancreatic cancer cell capable of being used for exosome tracing and application of the recombinant pancreatic cancer cell, and belongs to the technical field of biomedicine. The recombinant pancreatic cancer cell comprises a recombinant vector; a target gene pHl...
Gespeichert in:
Hauptverfasser: | , , , , , , , , |
---|---|
Format: | Patent |
Sprache: | chi ; eng |
Schlagworte: | |
Online-Zugang: | Volltext bestellen |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | The invention provides a recombinant pancreatic cancer cell capable of being used for exosome tracing and application of the recombinant pancreatic cancer cell, and belongs to the technical field of biomedicine. The recombinant pancreatic cancer cell comprises a recombinant vector; a target gene pHlurin M153R-CD63 is inserted into the recombinant vector, and the target gene pHlurin M153R-CD63 is used for According to the invention, a recombinant vector inserted with a target gene pHlurin M153R-CD63 is utilized to construct a pancreatic cancer stable cell line, and after extracellular exosomes and tail spots released in an extracellular matrix are effectively marked, the routing track of the exosomes can be more easily observed, so that the pathogenic behavior of migrated living cells is disclosed. The visual problem in exosome pathogenic behavior mechanism research is solved, the bright and stable fluorescent tracer material suitable for various cell culture environments is constructed, the exosome release an |
---|