Organ-like culture method and organ-like culture device

The invention provides a method for culturing an organoid, which comprises the following steps: carrying out division termination treatment on trophoblast cells to obtain treated trophoblast cells; in a culture container, performing adherent culture on the treated trophoblast cells to obtain adherent trophoblast cells; liquid gel containing organoid seed cells and hydrogel covers the trophoblast cells attached to the wall, the liquid gel is solidified, and an organoid growth gel layer is obtained; and adding a liquid-phase supplementary culture medium on the organoid growth gel layer to obtain a liquid-phase culture medium layer, and culturing the liquid-phase culture medium layer. The invention also provides an organ-like culture device. By means of the technical scheme, the method has the advantages in modeling efficiency, model stability, tumor organ cancer cell abundance maintenance and clinical relevance to drugs, and then a system closer to a human body is provided for drug screening and evaluation. 本发明