Method for preparing mesenchymal stem cells, mesenchymal stem cells and application
The invention provides a method for preparing mesenchymal stem cells (MSC), the mesenchymal stem cells and application. According to the method, trophoblast stem cells (TSC) are obtained by inducing EPSC differentiation, and mature and stable MSC is obtained by further inducing TSC differentiation,...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a method for preparing mesenchymal stem cells (MSC), the mesenchymal stem cells and application. According to the method, trophoblast stem cells (TSC) are obtained by inducing EPSC differentiation, and mature and stable MSC is obtained by further inducing TSC differentiation, so that the yield of MSC is greatly increased. The proliferation speed of the MSC obtained through differentiation is high, the multiplication time is about 1.5 days, the expression level of a surface marker of the mesenchymal stem cell is still kept normal after continuous passage is carried out for 16 times or more, and a large amount of EPSC-sourced MSC can be prepared through passage; in addition, the MSC prepared by the invention has the capability of secreting high-level FGF4 (Fibroblast Growth Factor 4), and is favorable for being developed into a cell therapeutic agent for treating type 2 diabetes mellitus.
本发明提供了一种制备间充质干细胞(MSC)的方法、间充质干细胞和应用。该方法通过诱导EPSC分化获得滋养层干细胞(TSC),进一步诱导TSC分化获得成熟稳定的MSC,大大提高了MSC产量。本发明分化获得 |
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